african green monkey kidney vero cells (ATCC)

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ATCC african green monkey kidney vero cells

Peptide from RNF213 truncation degrades ZIKV proteins and inhibits viral replication (A) The sequence of peptide from RNF213 truncation (PR-23) and control peptide. (B) A549 cells were infected with ZIKV (MOI = 0.01) and then treated with PR-23 (10 μM). Viral replication of cell supernatant was measured by plaque assay at 48 hpi. <t>(C–J)</t> <t>HEK293T</t> cells were treated with the indicated amount of PR-23 for 24 h, and then transfected with plasmids encoding HA-tagged ZIKV proteins. After 36 h, cell lysates were harvested and detected by western blot assay. GAPDH served as an internal control. (K) <t>Vero</t> cells were treated with the indicated dose of PR-23 for 6 h and transfected with the plasmid encoding GFP-tagged ZIKV E protein. After 36 h, the cells were fixed and subjected to immunofluorescence assay. The nucleus was stained with DAPI (blue). Images were obtained using a laser confocal microscope. Scale bars, 100 μm. Data are expressed as the mean ± SD from three independent experiments ( n = 3; ns, p > 0.05; ∗, p < 0.05; ∗∗∗, p < 0.001; ∗∗∗∗, p < 0.0001).

African Green Monkey Kidney Vero Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 18024 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/african+green+monkey+kidney+vero+cells/Vero/pmc13266186-240-9-19
Average 99 stars, based on 18024 article reviews

african green monkey kidney vero cells - by Bioz Stars, 2026-10

99/100 stars

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1) Product Images from "RNF213 isoform 2 restricts Zika virus through antiviral signaling and viral protein degradation"

Article Title: RNF213 isoform 2 restricts Zika virus through antiviral signaling and viral protein degradation

Journal: iScience

doi: 10.1016/j.isci.2026.116284

Figure Legend Snippet: Peptide from RNF213 truncation degrades ZIKV proteins and inhibits viral replication (A) The sequence of peptide from RNF213 truncation (PR-23) and control peptide. (B) A549 cells were infected with ZIKV (MOI = 0.01) and then treated with PR-23 (10 μM). Viral replication of cell supernatant was measured by plaque assay at 48 hpi. (C–J) HEK293T cells were treated with the indicated amount of PR-23 for 24 h, and then transfected with plasmids encoding HA-tagged ZIKV proteins. After 36 h, cell lysates were harvested and detected by western blot assay. GAPDH served as an internal control. (K) Vero cells were treated with the indicated dose of PR-23 for 6 h and transfected with the plasmid encoding GFP-tagged ZIKV E protein. After 36 h, the cells were fixed and subjected to immunofluorescence assay. The nucleus was stained with DAPI (blue). Images were obtained using a laser confocal microscope. Scale bars, 100 μm. Data are expressed as the mean ± SD from three independent experiments ( n = 3; ns, p > 0.05; ∗, p < 0.05; ∗∗∗, p < 0.001; ∗∗∗∗, p < 0.0001).

Techniques Used: Sequencing, Control, Infection, Plaque Assay, Transfection, Western Blot, Plasmid Preparation, Immunofluorescence, Staining, Microscopy

Modification:

Article Title: Ginsenoside Rh1 Suppresses Vesicular Stomatitis Virus Replication by Inhibiting Autophagy to Promote Immune Responses
Article Snippet: .. African green monkey kidney (Vero) cells (ATCC, CCL-81TM) were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, Carlsbad, CA, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS; Gibco, Carlsbad, CA, USA), 100 U/mL penicillin, and 100 μg/mL streptomycin. .. Caprine endometrial epithelial (EEC) cells, generously provided by Yongxi Dou (Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China), were cultured in Dulbecco’s minimal essential medium/Nutrient Mixture F-12 Ham’s medium (DMEM/F12, Carlsbad, CA, USA) containing 10% fetal bovine serum (FBS; Gibco), 100 IU/mL penicillin, and 10 μg/mL streptomycin.

Article Title: Targeting Host Metabolic and Epigenetic Rewiring Blocks Lytic Gammaherpesvirus Production
Article Snippet: .. Mouse fibroblast (NIH 3T3) cells (ATCC no. CRL-1658, Manassas, VA, USA) or African green-monkey kidney (Vero) cells (ATCC no. CCL-81, Manassas, VA, USA) were cultured at 37 °C and 5% CO 2 in complete Dulbecco’s Modified Eagle Medium (DMEM) containing high glucose, L-glutamine, sodium pyruvate (Genesee no. 25-500, El Cajon, CA, USA), 1% penicillin streptomycin (Genesee no. 25-512, El Cajon, CA, USA), and 10% serum. .. NIH 3T3 cells were maintained in 10% newborn calf serum (Thermo Fisher Scientific no. 16010159, Pittsburgh, PA, USA) and Vero cells were maintained in 10% fetal bovine serum (Genesee no. 25-550, El Cajon, CA, USA).

Article Title: ROP16 Promotes Epithelial‐Mesenchymal Transition‐Like Changes in Ocular Toxoplasmosis via STAT3 and TGF‐β1 Pathways
Article Snippet: The T. gondii strains used in the present study, including WH3 (wild‐type), WH3Δ rop16 , and WH6, were obtained from and are maintained by the Anhui Provincial Key Laboratory of Zoonoses. .. These strains were propagated in African green monkey kidney (Vero) cells (purchased from ATCC) and cultured in Dulbecco’s modified Eagle medium (DMEM) (Gibco) supplemented with 10% fetal bovine serum (FBS) (Hyclone). .. ARPE‐19 cells were cultured in DMEM/F‐12 medium (Gibco) supplemented with 10% FBS, penicillin (100 U/mL), and streptomycin (100 μg/mL) in a humidified 5% CO 2 atmosphere at 37°C.

Cell Culture:

Virus:

Article Title: SFV Replicon Vector Harbouring Porcine Epidemic Diarrhoea Virus Immunogens Delivered by Attenuated Salmonella Typhimurium Induces PEDV Neutralising Antibodies and Lactogenic Immunogenicity in BALB/c Mice.
Article Snippet: A total of 50 μg/mL of diaminopimelic acid (DAP) (Sigma-Aldrich, St. Louis, MO, USA) was added for the growth of ∆asd strains [23]. .. The tissue culture infective dose (TCID50) of the PED virus was propagated in African green monkey kidney (Vero) cells (ATCC CCL-81, Manassas, VA, USA), as described previously [24]. .. The synthetic genes encoding PEDV S1-NTD (19-223a.a) and N protein, codon optimised for eukaryotic expression, were commercially synthesised by Cosmogenetech (Seoul, Republic of Korea) based on consensus sequences derived from multiple alignments of 47 South Korean PEDV isolates (2010–2022) using ClustalW (EMBL-EBI).

Isolation:

Article Title: Molecular survey of mammalian orthoreovirus and Rotavirus A in Italian dogs and cats
Article Snippet: .. Viral isolation of MRV was attempted on African green monkey kidney (Vero) cells (ATCC CCL-81). ..

other:

Article Title: Triple-Mutated HSV-1 Expressing Soluble B7-1 Plus CTLA-4 Blockade Suppresses Lymph Node Metastasis in Tongue Cancer.
Article Snippet: African green monkey kidney (Vero) cells were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA).

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